il 21  (ATCC)

Journal: Advanced Science

Article Title: CD20 + FCRL4 + B Cells Activate CD8 + T Cells via MHC‐I Restriction in Nasopharyngeal Carcinoma Anti‐Tumor Immunity

doi: 10.1002/advs.202514982

Figure Lengend Snippet: Stimulation of B lymphoblastoid cell lines to differentiate into CD20 + FCRL4 + B cells in vitro. (A) Flow cytometry gating strategy for in vitro cultured cells. (B) Histograms showing the expression of functional molecules in B cell lines under different treatments (IFNγ 5 ng/mL; CD40L 100 ng/mL). (C)‐(D) Histograms showing FCRL4 expression in B cell lines under different treatments (LPS 5 µg/mL; SPA 5 µg/mL) with the proportion of FCRL4 − and FCRL4 + cells annotated. (E)‐(F) Bar chart comparing the expression levels of MHC‐I (E) and MHC‐II (F) molecules between the control group and the IFNγ‐treated group. ns P≥0.05; ** p<0.01. (G) Bar chart comparing the expression of FCRL4 molecules between the control group and the CD40L intervention group. * p<0.05. (H) Connected line plot comparing the expression levels of MHC‐I molecules between FCRL4 + and FCRL4 − cells in the same sample after co‐treatment with IFNγ and CD40L. ** p <0.01. (I) Bar chart showing the proportion of FCRL4 + cells in B cells in the control group, LPS‐treated group, and SPA‐treated group. * p <0.05; *** p <0.001; **** p <0.0001. (J) The bar chart compares the signal intensity of FCRL4 expression in ARH‐77 cells in the blank control group, HK‐1 co‐culture group, and HK‐1 lysate stimulated group. ns P≥0.05; ** p <0.01; *** p <0.001.

Article Snippet: Following 24 h of stimulation with the following reagents, either individually or in combination: 5 ng/mL human IFNγ (Beyotime, P5664), 100 ng/mL soluble CD40L (CST, #32621), 20 ng/mL LPS (Abiowell, AWH0796), 20 ng/mL SPA (Sigma‐Aldrich, P6031), and 10 μg/mL NPC‐lysate, flow cytometry analysis or an antigen peptide uptake experiment was conducted.

Techniques: In Vitro, Flow Cytometry, Cell Culture, Expressing, Functional Assay, Control, Co-Culture Assay